Application of Immunohistochemistry for Pathogen Detection in Fishes

Abstract

Fishes, namely,Catlacatla (150 ± 20 g) were reared in laboratory conditions, and were fed with the artificial diet. After 4 weeks of acclimatization, fishes were immunized intraperitoneally with bovine serum albumin (BSA),a protein antigen. After antigen administration, spleen samples were dissected out aseptically from the fish on weakly intervals, i.e., on days-7, 14, 21 and 28. The spleen samples were diagnosed by immuno-electron microscopy for the presence of BSA antigen. From the results it was observed that the antigen/pathogen can be definitely located from the fish tissue at early stages of infection