Abstract:
An innovative fluorescein appended naphthalene diimide based probe (FANDI) has been prepared and
characterized to selectively recognize hypochlorite or ClO− ions in the presence of other reactive oxygen
species (ROS) and biorelevant ions, using a unique chemodosimetric method. Hypochlorite induced oxi dation can efficiently alter the initial photophysical properties of FANDI and shows an easily detectable
“turn on” green fluorescence. The chemodosimeter FANDI can efficiently detect exogenous as well as
endogenous ClO− ions in RAW 264.7 cells (macrophages) and zebrafish embryos (Danio rerio) which
further ensures the high potential, easy cell permeability and photostability of FANDI and makes it worth
exploring in the future
