Physiological relevance of nitric oxide in ovarian functions: An overview

Abstract

Nitric oxide (NO, nitrogen monoxide), a short-lived, free radical carrying an unpaired electron, is one of the smallest molecules synthesized in the biological system. In addition to its role in angiogenesis, neuronal function and inflammatory response, NO has wide-spread significance in regulation of ovarian function in vertebrates. Based on tissue-specific expression, three different nitric oxide synthase (NOS) isoforms, neuronal (nNOS) or NOS1, inducible (iNOS) or NOS2 and endothelial (eNOS) or NOS3 have been identified. While expression of both inducible (iNOS) and constitutive NOS (eNOS) isoforms varies considerably in the ovary at various stages of follicular growth and development, selective binding of NO with proteins containing heme moieties have significant influence on ovarian steroidogenesis. Besides, NO modulation of ovulatory response suggests physiological significance of NO/NOS system in mammalian ovary. Compared to the duality of NO action on follicular development, steroidogenesis and meiotic maturation in mammalian models, participation of NO/NOS system in teleost ovary is less investigated. Genes encoding nos1 and nos2 have been identified in fish; however, presence of nos3 is still ambiguous. Interestingly, two distinct nos2 genes, nos2a and nos2b in zebrafish, possibly arose through whole genome duplication. Differential expression of major NOS isoforms in catfish ovary, NO inhibition of meiosis resumption in Anabas testudineus follicle-enclosed oocytes and NO/sGC/cGMP modulation of oocyte maturation in zebrafish are some of the recent advancements. The present overview is an update on the advancements made and shortfalls still remaining in NO/NOS modulation of intercellular communication in teleost vis-à-vis mammalian ovary.